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ppka  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ppka
    (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 <t>(I)</t> <t>PKA</t> (J) and <t>pPKA</t> (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).
    Ppka, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ppka/Phospho-PKA+C+(Thr197)+Rabbit+mAb/bio_rxiv__2025__11__20__689524-243-54-55
    Average 95 stars, based on 194 article reviews
    ppka - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Calcitonin-Driven Reactivation of the Hippo Tumor-Suppressor Cascade Attenuates YAP/TAZ Oncogenic Signaling in Glioblastoma"

    Article Title: Calcitonin-Driven Reactivation of the Hippo Tumor-Suppressor Cascade Attenuates YAP/TAZ Oncogenic Signaling in Glioblastoma

    Journal: bioRxiv

    doi: 10.1101/2025.11.20.689524

    (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 (I) PKA (J) and pPKA (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).
    Figure Legend Snippet: (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 (I) PKA (J) and pPKA (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).

    Techniques Used: Injection, In Vivo, Staining, Confocal Microscopy

    Related Articles

    other:

    Article Title: Substance P and adenosine signaling pathways regulate exosomal sorting of miR-21 in colonic epithelial cells
    Article Snippet: Reagents used are as follows: protease inhibitors cocktail (Cell Signaling Technology, MA, USA), Penicillin-Streptomycin (Cat.# P4333; Sigma-Aldrich), exosome-depleted fetal bovine serum (Life Technologies, Carlsbad, CA), fetal bovine serum (Cat.# F2442; Sigma-Aldrich), M3D media (Incell, San Antonio, TX), advanced DMEM/F-12 (Cat.# 12,634–010; Invitrogen), DMEM (Cat.# D5796; Sigma-Aldrich), Y-27632 (Cat.# 1254; R&D Systems), SB 431542 (Cat.# 1614; R&D Systems), PSB-1115 (Cat.# 2009; Tocris Bioscience), siTUT7 and siControl (Ambion), Lipofectamine RNAiMAX (ThermoFisher), Matrigel (Cat.# 356234; BD Biosciences), anti-actin (1:1,000, A2066; Sigma Aldrich), anti-GAPDH (1:1,000, 5174S; Cell signaling Technology), anti-CD9 (sc-13118; Santa Cruz Biotechnology, Dallas, TX), anti-A33 (sc-398702; Santa Cruz Biotechnology), sCREB (1:1,000, 4820S; Cell signaling Technology), anti-p-CREB (1:1,000, 9198S; Cell signaling Technology), anti-pPKA (1:1,000, 5661S; Cell signaling Technology), anti-TUT7/anti-ZCCHC6 (1:500, 25196-1-AP; Proteintech).

    Article Title: The Interplay of Protein Kinase A and Perilipin 5 Regulates Cardiac Lipolysis
    Article Snippet: Blotted proteins were probed using the following primary antibodies: anti-GAPDH (catalog no. 2118, Cell Signaling Technology, Boston, MA); anti-His (catalog no. 27-4710-01, GE Healthcare); anti-HSL (catalog no. 4107, Cell Signaling Technology); anti-pHSL (catalog no. 4126, Cell Signaling Technology), and anti-pPKA (catalog no. 4781, Cell Signaling Technology).

    Article Title: The Interplay of Protein Kinase A and Perilipin 5 Regulates Cardiac Lipolysis*
    Article Snippet: Blotted proteins were probed using the following primary antibodies: antiGAPDH (catalog no. 2118, Cell Signaling Technology, Boston, MA); anti-His (catalog no. 27-4710-01, GE Healthcare); antiHSL (catalog no. 4107, Cell Signaling Technology); anti-pHSL (catalog no. 4126, Cell Signaling Technology), and anti-pPKA (catalog no. 4781, Cell Signaling Technology).

    Article Title: Binding of FUN14 Domain Containing 1 With Inositol 1,4,5-Trisphosphate Receptor in Mitochondria-Associated Endoplasmic Reticulum Membranes Maintains Mitochondrial Dynamics and Function in Hearts in Vivo
    Article Snippet: Anti-CREB (#4820), Anti-pCREB-Ser133 (#9198), Anti-VDAC1 (#4661), anti-pDRP1-Ser616 (#3455), anti-LC3 (#4108), anti-PDI (#2446), anti-Cytochrome c (#4272), anti-pPKA (#4781), anti-βTubulin (#2146), and anti-GFP (#2555) were purchased from Cell Signaling Technology Inc. (Danvers, MA).

    Western Blot:

    Article Title: ZIP13 regulates lipid metabolism by changing intracellular iron and zinc balance
    Article Snippet: .. The following antibodies were used for immunoblotting: anti-Fth1 (1:1,000; Abcam, St. Charles, MO, #EPR3004Y), anti-FTL (1:1,000; Abcam, #ab69090), anti-IRP2 (1:1,000; Millipore, Bedford, MA, USA, #MABS2030), anti-TfR (1:1000; Invitrogen, Carlsbad, CA, USA, #136890), anti-actin (1:5000, Sigma, #A2228), anti-pPKA (1:1000; Cell Signaling, Danvers, MA #5661), PKA (1:1000; Cell Signaling, #5842) and anti-GAPDH (1:3,000; Cell Signaling, #2118). ..



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    (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 <t>(I)</t> <t>PKA</t> (J) and <t>pPKA</t> (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).
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    Image Search Results


    (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 (I) PKA (J) and pPKA (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).

    Journal: bioRxiv

    Article Title: Calcitonin-Driven Reactivation of the Hippo Tumor-Suppressor Cascade Attenuates YAP/TAZ Oncogenic Signaling in Glioblastoma

    doi: 10.1101/2025.11.20.689524

    Figure Lengend Snippet: (A) 0.15×10 6 MGG4-Luc GSCs were injected intracranially into the brain of nude mice. The tumor was allowed to grow for seven days, after which CT was administered intranasally at a dose of 2 IU/ animal. Representative images of the animals’ in vivo bioluminescence were taken every five days from the date of injection. ( B) The average radiance efficiency between the PBS and CT-administered groups is plotted (two-way ANOVA; n=8 in PBS group and n=7 in CT-treated group). ( C) The Kaplan-Meier graph shows the survival difference between the two groups of mice that were administered PBS or CT. ( D) Haematoxylin and Eosin staining shows a larger tumor (depicted by dark blue colour) in animals administered with intranasal PBS compared to a smaller tumor in the CT-administered group. Confocal microscopy shows level of YAP (E) , TAZ (F), pYAP/TAZ (G), LATS1 (H) pLATS1 (I) PKA (J) and pPKA (K) levels in the PBS and CT-treated groups (Magnification-63x, scale bar-20µm).

    Article Snippet: The primary antibodies used in the study were purchased from the following companies: CTR (Invitrogen PA5-25594, 1:1000), YAP (CST 14074S, 1:1000), TAZ (CST 4883S, 1:1000), pYAP (CST 4911S, 1:1000), CTGF (CST 86641S, 1:1000), LATS1 (CST 9153S, 1:500), pLATS1 (CST 8654S 1:500), MST1 (CST 3682S, 1:1000), pMST1 (CST 49332S), GFP (CST 2956S), PKA (CST4782S, 1:1000), pPKA (CST 5661S, 1:1000), SOX2 (Abcam ab92494, 1:2000), GAPDH (SIGMA G8795, 1:25000).

    Techniques: Injection, In Vivo, Staining, Confocal Microscopy

    Journal: Oncotarget

    Article Title: Imipridones ONC201/ONC206 + RT/TMZ triple (IRT) therapy reduces intracranial tumor burden, prolongs survival in orthotopic IDH-WT GBM mouse model, and suppresses MGMT

    doi: 10.18632/oncotarget.28707

    Figure Lengend Snippet:

    Article Snippet: pPKA substrate , Cell Signaling , 9621 , 1:1000.

    Techniques: Concentration Assay